Anthelmintic effect and phytochemical assay of selected grasses against Fasciola spp. in water buffalo (Bubalus bubalis [manuscript]
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Date
2019
Authors
Arjay B. Reyes
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Abstract
This study aimed to determine the effect of Guinea grass (Panicum maximum), Napier grass (Pennisetum purpureum) and Para grass (Brachiaria mutica) extract against adult liver flukes in buffaloes. Aqueous ethanol extract (10mg/ml) such grasses were prepared for anthelmintic trial against Fasciola spp. in buffaloes. In all the treatments, Guinea grass leaf extract caused a significant mortality against adult liver flukes with percent mortality of 33.33%. Napier grass leaf extracts has a mean mortality of 26.67% has comparable anthelmintic effect with Guinea grass. On the other hand, para grass at 6.67% showed significantly lower fluke mortality than Guinea grass and Napier grass. All the plant treatments are not comparable with the positive control which had a mortality of 100%. Histopathological changes such as vacuole formation, damage parenchyma and disruption of tegument were seen under microscope. Phytochemical screening of Guinea grass leaf extract showed presence of saponins, this phytochemical constituent is responsible in the anthelmintic action of the extract. These findings indicate that the Fasciola spp. is vulnerable to selected grasses.