Theses and Dissertations

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Browsing Theses and Dissertations by Adviser "ARMAN M. PARAYAO, Ph.D."

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    Antimicrobial resistance profile of (Enterococcus sp. and Escheria coli isolated from soil samples in selected carabao (bubalus bubalis) farms in Rizal and General Mamerto Natividad, Nueva Ecija
    (2019) Ellyza Marie F. Cabog
    Enterococcus sp. from the family of Enterococcaceae, is a gram positive cocci formerly known as a group D streptococci. It can be found on the intestinal tract of human, animals and also in soil. Whereas, Escherichia coli is a rod shaped, gram negative bacteria under the family Enterobacteriaceae. They are facultative anaerobic that does not require oxygen, but grows better in the presence of oxygen. This study was conducted to determine the antimicrobial resistance profile of Enterococcus sp. and E. coli. from soil samples in selected carabao farms. Different cultures media such as Bile Esculin Agar, MacConkey Agarand Eosin Methylene Blue Agar were utilized for the isolation of bacteria and VITEK. 2 System was used for the identification and determination of antimicrobial resistance profile. The results revealed that Enterococcus foercium, Enterococcus durans and Enterococcus hirae isolated in soil samples were sensitive to 13 antibiotics, resistant in three antibiotics and some are intermediate to Erythromycin and Nitrofurantoin. Whereas, all E. coli isolates were sensitive towards 37 different antimicrobial agents used and have a low percentage of resistance to antibiotics.
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    Microbiological assessment and characterization of bacteria associated with dairy carabao's milk based (Bibingka)
    (2019) Junnida B. Miranda
    Foodborne microorganisms can lead to serious outbreaks in the community, hence food safety is vital for the consumer. This study was focused on microbiological assessment and characterization of bacteria associated with dairy carabao's milk-based bibingka sold in local dairy cooperatives. Physical assessment of the product was performed prior the bacteriological assessment. Conventional method and Petrifilm tm was used in enumerating microorganisms. Aerobic plate count, presumptive confirmatory and completed coliform and E. coli enumeration were utilized for the conventional method while Petrifilm Anaerobic Count Plate and Petrofilm tm E. coli/ Coliform Count Plate was used in 3M Petrifilm. The physical assessment of the product showed no objectionable traits in terms of color, color, appearance and taste. Results showed that within the two samplings, the dairy carabao's milk-based bibingka satisfied some of the given standards for its properties. Parallel results obtained among conventional methods and Petrifilm tm for analyses of aerobics, total coliforms and Escherichia coli. Three isolates were the food-prevalent bacteria that appeared in both samplings. three Gram positive bacterial isolates were characterized based on its cultural and morphological characteristics.