Post-mortem goat testicle cooled handling condition and its influence on the quality of epididymal sperm [manuscript]
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Date
2017
Authors
John Evander V. Francisco
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Abstract
The general objective of this study was to evaluate and determine the influence of cooled temperature (17-22˚C). Sperms were recovered from the incised epididymal tissue either after 4 hours (T1) and 24 hours (T2) post-mortem. The epididymal sperms were processed for cryopreservation using TCLR buffer solution supplemented with 20% (v/v) egg yolk and 7% (v/v) glycerol. Microscopic evaluations including determination of motility, livability, and morphology were done on the freshly recovered and processed epididymal sperm before (pre-freeze/extended Sperm) and after cryopreservation (post-thaw sperm). In addition, sperm volume and concentration, as well as weights of testicles and cauda epididymal sperm before (pre-freeze/extended sperm) and after cryopreservation (post-thaw Sperm). In addition, sperm volume and concentration, as well as weights of testicles and cauda epididymal tissue, were determined. The findings of the study revealed that there was a significant reduction in sperm concentration(p<0.05) but not on sperm volume (p>0.05) after 24 hours post-mortem. In the freshly recovered epididymal sperm, the percentage motility was not significantly different (p>0.05) between treatments. However, there was a significant (p<0.05) decreased in the percentage livability and increased in the percentage abnormality overtime (after 24 hours). On the other hand, pre-freeze epididymal sperms between treatments showed no significant difference (p>0.05) in any parameter, except for motility (p<0.05). The cryopreserved epididymal sperm recovered after 4 hours obtained a mean post-thaw motility of 10.63%, livability of 40.38% and abnormality of 50.09%, whereas the pre-freeze quality of epididymal sperms recovered after 24 hours did not quality for further freezing. No significant difference (p>0.05) was observed in ther testicular parameters between treatments.