Isolation and characterization of the gonadal primordial germ cells (gPGCs) of Guinea fowl (Numida meleagris) from 7-9 day old embryos [manuscript]
| dc.contributor.author | Nikko M. Pardua | |
| dc.date.accessioned | 2026-04-28T00:18:15Z | |
| dc.date.available | 2026-04-28T00:18:15Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | The study aimed to isolate and characterize the gonadal primordial germ cells (gPGCs) of guinea fowl. Specifically, the study determined the embryonic age that optimally released gPGCs, the embryonic age that dischrged the highest number of gPGCs and morphological characteristics of the gPGCs. The right and left gonads collected from the 7 and 9 days old guinea fowl embryos were separately placed in a petri dish containing 500 µl of phosphate buffered saline without Mg2+ and Ca2+ (PBS-). The petri dishes were placed in an incubator maintained at 38.5°C and subsequently observed at 3 hours of incubation. Results showed that the 9-day old guinea fowl embryos released the highest yield of discharged gPGCs collected. It is the optimum incubation age to isolate and characterize the gPGCs of the guinea fowl embryos. It was also found out that the left gonad had more number of released gPGCs than the right. The observed gPGCs were round shaped cells with large central nucleus. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/1188 | |
| dc.language.iso | en_US | |
| dc.relation.supervisor | VIRGILIO D. VIERNES, DVSM, Ph.D. | |
| dc.title | Isolation and characterization of the gonadal primordial germ cells (gPGCs) of Guinea fowl (Numida meleagris) from 7-9 day old embryos [manuscript] | |
| dc.type | Thesis |