Detection of Burkholderia pseudomallei using polymerase chain reaction (PCR) in sheep (Ovisaries) in Nueva Ecija [manuscript]
| dc.contributor.author | Dennis O. Sayco Jr | |
| dc.date.accessioned | 2026-04-27T06:55:54Z | |
| dc.date.available | 2026-04-27T06:55:54Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Melioidosis is a bacterial disease that affects humans and many species of animals including sheep. It is systemic disease that may affect almost any organ in an affected animal (Benoit et al., 2015) and a disease of public health importance in southeast Asia that is associated with high case-fatality rates in animals and humans (Cheng et al., 2005). The study was performed to detect Burkholderia pseudomallei from nasal swab of sheep around Nueva Ecija using Polymerase Chain Reaction/ A total of 40 sheep that are randomly picked at Nueva Ecija were collected with nasal swab samples. Extracted samples were subjected to bacterial culture and DNA extraction. Identification of Burkholderia pseudomallei was molecularly confirmed through Polymerase Chain Reaction with a primer of 16S rRNA gene and Tat domain gene primer and were subjected to gel analysis for identification. Results showed that all 40 samples were negative from 16S rRNA gene and Tat domain gene primer that was used for the detection of Burkholderia pseudomallei. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/1167 | |
| dc.language.iso | en_US | |
| dc.relation.supervisor | JAYLORD M. PIOQUINTO, DVM | |
| dc.title | Detection of Burkholderia pseudomallei using polymerase chain reaction (PCR) in sheep (Ovisaries) in Nueva Ecija [manuscript] | |
| dc.type | Thesis |