Mycochemical profiling and biological activities of fungi (Aspergillus niger and Rhizomucor pusillus) associated with vermicast [manuscript]
| dc.contributor.author | Jamille B. Sabado | |
| dc.date.accessioned | 2025-10-22T00:53:06Z | |
| dc.date.available | 2025-10-22T00:53:06Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | This study was undertaken to determine the mycochemical composition of fungi associated with vermicast namely; Aspergillus niger and Rhizomucor pusillus. Their antibacterial potential as protectant and eradicant and their ability to scavenge free radicals or their DPPH radical scavenging activity were also evaluated. Mycochemical screening of A. niger and R. pusillus ethanolic extract and fungal spent revealed the presence of flavonoids and terpenoids. Tannins was only detected in the A. niger ethanolic extract. In addition, alkaloids was only observed in R. pusillus ethanolic extract. For the antibacterial activity as protectant, at 12 and 24 hours of incubation, the least zone of colonization of S. aureus and E. coli were recorded in plates treated with A. niger ethanolic extract (9.64 mm and 12.35 mm for S. aureus; 9.60 mm and 12.37 mm for E. coli) and R. pusillus ethanolic extracts (0.00 mm and 13.03 mm for S. aureus: 19.35 mm and 32.44 mm for E. coli). On the other hand, for the antibacterial activity as eradicant, zone of inhibition at 12 and 24 hours of incubation against the bacterial pathogens were recorded in ethanolic extract of A. niger (9.43 mm and 9.28 mm in S. aureus) and R. pusillus (11.94 mm and 11.75 mm in S. aureus; and 9.40 mm and 9.08 mm in E. coli). Lastly, for the DPPH radical scavenging activity, among the four extracts, A. niger ethanolic extract recorded the highest ability to scavenge free radicals with 73.03% followed by R. pusillus ethanolic extract with 71.86% A. niger spent and R. pusillus fungal spent were recorded to be the least with percentage of 45.20% and 29.96% respectively. The present study indicates that the secondary metabolites of the extracts can be act as source of wide spectrum antibiotics and antioxidant. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/788 | |
| dc.language.iso | en | |
| dc.relation.supervisor | MARY JHANE G. VALENTINO, M.Sc. | |
| dc.title | Mycochemical profiling and biological activities of fungi (Aspergillus niger and Rhizomucor pusillus) associated with vermicast [manuscript] | |
| dc.type | Thesis |