Molecular characterization of the porcine reproductive and respiratory syndrome (PRRS) field strain virus and the commercial PRRS vaccine virus from grower-finisher Pigs [manuscript]
| dc.contributor.author | Donalyn S. Vigilia | |
| dc.date.accessioned | 2026-04-27T06:57:25Z | |
| dc.date.available | 2026-04-27T06:57:25Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | The study established the difference betwen the PRRS vaccine virus from the PRRS field isolate through molecular characterization Representative RT-PRC amplified products from blood, semen aborted fetus, lungs, and tonsils of non-vaccinated and PRRS vaccinated pigs were sent for gene sequencing in order to generate a phylogenetic tree of the PRRS virus. Among the 58 animals that had history of PRRS, 38 resulted positive for RT-PCR Specifically, 12 out 26 from vaccinated animals resulted positive and 26 out of 32 from non-vaccinated animals tested positive for PRRS virus. Phylogenetic analysis showed that the sequence of the non-vaccinated field isolates clustered together showing a slight difference from the vaccine virus (Ingelvac) and the VR2332, a clone sequence reference of the North America strain which was a highly virulent and a 2008 isolate from China (FJ175689.1). On the other hand, it was observed that the published sequence of the European PRRS isolates was distant among the sequences on the non-vaccinated field isolates. Hence, the field isolates from non-vaccinated pigs in the Philippines belonged to the North American strain. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/1168 | |
| dc.language.iso | en_US | |
| dc.relation.supervisor | CLARISSA YVONNE J. DOMINGO, DrPH | |
| dc.title | Molecular characterization of the porcine reproductive and respiratory syndrome (PRRS) field strain virus and the commercial PRRS vaccine virus from grower-finisher Pigs [manuscript] | |
| dc.type | Thesis |