Molecular characterization of the porcine reproductive and respiratory syndrome (PRRS) field strain virus and the commercial PRRS vaccine virus from grower-finisher Pigs [manuscript]
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Date
2019
Authors
Donalyn S. Vigilia
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Abstract
The study established the difference betwen the PRRS vaccine virus from the PRRS field isolate through molecular characterization Representative RT-PRC amplified products from blood, semen aborted fetus, lungs, and tonsils of non-vaccinated and PRRS vaccinated pigs were sent for gene sequencing in order to generate a phylogenetic tree of the PRRS virus. Among the 58 animals that had history of PRRS, 38 resulted positive for RT-PCR Specifically, 12 out 26 from vaccinated animals resulted positive and 26 out of 32 from non-vaccinated animals tested positive for PRRS virus. Phylogenetic analysis showed that the sequence of the non-vaccinated field isolates clustered together showing a slight difference from the vaccine virus (Ingelvac) and the VR2332, a clone sequence reference of the North America strain which was a highly virulent and a 2008 isolate from China (FJ175689.1). On the other hand, it was observed that the published sequence of the European PRRS isolates was distant among the sequences on the non-vaccinated field isolates. Hence, the field isolates from non-vaccinated pigs in the Philippines belonged to the North American strain.