Molecular identification of wild macrofungi in Mt. Arayat San Juan Baño, Arayat Pampanga
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Date
2019
Authors
John Ronald T. Policarpio
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Abstract
The classical way of identifying macrofungi is by using morphological means but because of the similarities in morphology when nearing the genus and species level of classification makes identification through morphological means difficult. In this study the efficiency and accuracy of identification through molecular means is highlighted. The collected wild macrofungi were subjected to DNA extraction using a modified CTAB method and amplified using Internal Transcribed Region (ITS). The PCR products are then sent to Apical scientific sequencing in Selangor, Malaysia for sequencing and used CLUSTALW in MEGA7 for alignment. BLAST analysis revealed the identity of the samples to be JRPI m(Schizophyllum commune) with a 100% identity, JRP2 (Hexagonia tenus) with 97% identity, JRP3 (Fulvifomes fastuosus) with a 99% identity, JRP4 (Trametes cubensus) with 99% identity, JRP5 (Psathyella cacao) with a 97% identity, JRP6 (Geastrum morganii) with 99% identity, JRP7 (Ganoderma lucidum) with 99% identity, JRP8 (pleurotus djamor) with a 99% percent identity, JRP9 (Marasmius tricolor) with 96% identity and JRP10 (Neonothopanus nambi) with 98% identity with similar species deposited at Genbank.