Effect of Lentinus strigosas ethanol extract on food intake and locomotion of N2 wild strain (Caenorhabditis elegans)
| dc.contributor.author | Yusela Kris DC. Aquino | |
| dc.date.accessioned | 2025-10-22T06:18:22Z | |
| dc.date.available | 2025-10-22T06:18:22Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Lentinus strigosus is a wood-rotting edible mushroom that grow mostly in cluster. To determine its potential as far reducing agent, the fruiting body ethanol extract was tested in Caenorhabditis elegans. Nematode lethality test, pharyngeal behavior assay, and locomotion assay were conducted. Lethality test has shown that the extract is slightly toxic. Increasing extract concentration increases toxicity, however, the survival rates are not significantly different. Pharyngealpumping decreases as extract concentration increases. The lowest pumping rate was recorded at 1000 ug/ml (12.78 pumps per minute (ppm) and highest at 10 ug/ml (15.64 ppm) at 48 hours postreatment application while the lowest was recorded at 1000 ug/mL (6.17 ppm) and highest at 10 ug/mL (15.64 ppm) at 120 hours post treatment application. Locomotion increases as extract concentration increases. Acute locomotion assay shows that the dwelling locomotion of the nematodes was highest at 1000 ug/ml. (6.99 reversals per minute) and lowest at 10 ug/mL (3.38 reversals per minute). The roaming locomotion at the nematodes was highest at 1000 ug/mL (48.58 body bends per minute (bbpm) and lowest at 10 ug/mL (37.53 bbpm). In the chronic locomotion assay, the dwelling locomotion of the nematodes was highest at 1000 ug/mL (4.52 reversals per minute) and lowest at 300 ug/mL (3.43 reversals per minute). Meanwhile, the roaming locomotion of the nematodes was highest at 1000 ug/mL (30.93) and lowest at 10 ug/mL (23.16 bbpm). These results may be the start of the discovery of a new less-invasive, more cost effective treatment to obesity. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/801 | |
| dc.language.iso | en | |
| dc.relation.supervisor | RICH MILTON R. DULAY, M.Sc. | |
| dc.title | Effect of Lentinus strigosas ethanol extract on food intake and locomotion of N2 wild strain (Caenorhabditis elegans) | |
| dc.type | Thesis |