Gene expression linked to antivirulence activity of the Ilongot-Egongot Ethnobotanicals from Bayanihan, Maria Aurora, Aurora against biofilm formation in Pseudomonas aeruginosa [manuscript]
Date
2018
Authors
Rebecca I. Santos
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Abstract
With the widespread appearance of antibiotic resistant bacteria, demand for novel strategies to control infectious diseases is greatly increasing. The discovery that the use of quorum-sensing (QS) system is highly significant in many bacteria to coordinate virulence such as biofilm development has indicated an advanced and promising target for antimicrobial drugs.
Ethanolic extracts of selected Philippine Ilongot-Egongot ethnobotanicals were screened for their quorum sensing inhibition activity biofilm formation in P. aeruginosa clinical isolate and P. aeruginosa BIOTECH 1335: Stachytarpeta jamaicensis, Adenanthera intermedia (leaves and seeds), Mikania micarantha, Hyptis suaveolens, Premna odorata, Cymbopogan winterianus, Phyllanthus urinaria, Dillenia philippinensis, Hydrocotyle vulgaris, Senna alata, Urena lobata, Ceiba pentandra, Ficus sp., Eleusine indica, Diplazium esculentum and Talahib (no known scientific name).
Seven ethanolic extracts namely M. micrantha, H. suaveolens flowers, H. vulgaris, A. intermediate, E. indica leaves, D. esculentum and talahib have inhibitory effect on P. aeruginosa clinical isolate biofilm formation. Moreover, significant decrease in biofilm formation was shown in twelve of the ethanolic extract against P. aeruginosa BIOTECH 1335. These were S. jamaicensis., Mikania micrantha, H. suaveolens flowers, H. vulgaris, U. lobata, C. pentandra, A. intermedia, E. indica roots, E. indica seeds, D. esculentum, H. suaveolens leaves and Talahib.
Three out of four plant extracts namely M. Micrantha, talahib and A. intermedia significantly downregulated the lasR in the P. aeruginosa clinical isolate and P. aeruginosa BIOTECH 1335. This indicate the potential of these ethnobotanicals for therapeutic approach in inhibiting bacterial virulence, without developing resistance.