Microbial isolation and characterization of carabao-based product (espasol de leche) sold in Dairy Cooperatives of Nueva Ecija [manuscript]
Date
2018
Authors
Lois Marie A. Salmorin
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Abstract
The detection and identification of food borne pathogens found in surfaces that come into contact with food are an important component of any integrated program to ensure the safety of foods throughout the food supply chain. The study was conducted to isolate and characterize different bacterial organisms present in Espasol de leche sold in dairy cooperatives of Nueva Ecija.
Espasol deleche was collected from a dairy cooperative in Nueva eCija during the month of March and April and was evaluated using standard plate count as well as the presumptive test for the presence of E. coli and coliforms. sampling was performed three times, with each sampling observed during the freshly produced (day0) and during its 2nd day of storage.
The standard plate count of Espasol de leche in cfu/g, within three samplings have all satisfied the given standards for ready to eat foods. No growth of presumptive Enteric bacteria and coliforms were also observed. It was concluded that the product was under the @Good@ category of microbiological quality.
Distinct bacterial colonies were then isolated and were observed based on its morphological and cultural characteristics. A total of five bacterial isolates were subjected to one point inoculation, nutrient broth, Gram staining, and biochemical testing.
Results showed that three bacterial colonies were Gram positive and two were Gram negative. Using API 20 Et system of identification, three bacterial isolates were identified as Pseudomonas aeruginosa, Pasteurella pneumotropical/Mannheimia haemolytica, and Pseudomonas luteola which are mostly contaminants found in the environment. Routine quality check for conformance to good handling and manufacturing practices of dairy cooperatives are highly recommended.