Isolation and characterization of waterborne bacteria from CLSU water system and their resistance to selected plant extracts
Date
2019
Authors
Rocelle Jirah C. Hufana
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Abstract
Plant materials are readily available and cost in far less than antibacterial agents. Due to this, this study aimed to characterize the waterborne bacteria that were isolated based on its morphological and cultural characteistics and to determine the antibacterialproperties of selected allergenic grasses, namely Cynodon dactylon (L.) Pers. (Bermudagrass), and Saccharium spontaneum L. (Wild sugarcane) against the siolated and characterized water borne bacteria.
A total of two bacterial isolates were pure cultures and were then subjected to morphological and cultural characterization. based on the result of the characterization and rapid testing, the colonies were identified as Myroides spp. and Escherichia coli. The plant samples were extracted through hexane and ethanol solvents. The efficacy of the plant extracts was tested against the isolated bacteria through disc diffusion assay. Although zones of inhibition were observed, statistical analysis shows that the extracts are not effective as eradicant against the two siolated waterborne bacteria. This might be suggested that the inhibitory activities of the plant extracts were found to be exact and dose dependent.