Physico-chemical characterization and microbial isolation of bacteria associated with pasteurized buffalo milk from a Dairy Cooperative in Nueva Ecija [manuscript]
Date
2018
Authors
Kathleen Olivia B. Martin
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Abstract
Pasteurized buffalo milk from a dairy cooperative in Nueva Ecija was evaluated through its physico-chemical properties and microbial load. Organoleptic tests were done alongside with the four platform tests namely, alcohol precipitation, lactometer, titratable acidity and clot on boiling tests. The microbial load of the milk was determined through standard plate count. After three samplings, the study concluded that within the freshly pasteurized state of the milk it satisfies all the given standard, its second and fifth dat state shows that the milk is not fit consumption since it failed to satisfy the given standards. Presence of enteric bacteria was detected on two samplings, however on the third sampling, the enteric bacteria was not detected on two samplings, however on the third sampling the enteric bacteria was not detected, this may be attricuted to the ocular visit done at the dairy cooperative before the third sampling. Isolates were produced and characterized by conventional method. It was found that Gran negative organisms dominated the pasteurized milk sample and biochemical analysis using API 20 E revelaed that these isolates belongs to genus. Stenotrophomonas, Chryseobacterium, Pantoea, Pseudomonas and Cronobacter.