Molecular identification of freshwater microalgae from the waterfalls of Paracelis, Mt. Province.
Date
2019
Authors
Larny O. Martin
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Abstract
Microalgae, is diverse and the species is present in all existing earth ecosystems. The distribution of microalage are everywhere and be found in saltwater, brackish or freshwater. The study focused on the isolation and cultivation of microalgae, describe the morphological characteristic and identification of the microalgae strain through molecular technique. Three species of microalage was isolated from the three waterfalls of Paracelis, Mt. Province namely Canabo Falls, Iyo Falls and Maalinsawang Falls. Isolated microalgae from each falls was labelled as CS2, MF1 and IF. Microalga strain from CS2 is spherical, MF1 isolate is thread -like shape, while IF strain is sickle-shape. All the isolate color is green. Through molecular technique the isolated microalgae is identify, using the 16s rRNA and rbcL. The CS2 amplified by using rbcL gene marker and MF1 was amplified using 16S gene marker. While IF microlaga strain was not amplified at 16S rRNA and rbcL. BLAST analysis of the sample showed that CS2 has 100% similarity to Micractinium sp. and MF1 has a 92.12% similarity Leptolyngbya laminosa with similar samples deposited at Genbank. The similarity of the morphological characters of the two supports the successful molecular identification of the microalgae with the use of 16S and rbcL gene markers.