Antiproliferative and cytotoxic activity of Morinda citrifolia against human cancer cell lines using MTT assay
Date
2019
Authors
Jahziel M. Culala
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Abstract
Morinda citrifolia, commonly called as Noni, has been reported to have a wide range of health benefits for cancer, infection, diabetes, astma, hypertension and pain. Initial pharmacological studies on M. citrifolia have focused on cancer, inflammation and metabolic diseases, hence this study determined its potential anti-proliferative and cytotoxic activity against human cancer cell lines: breast adenocarcinoma cell line (MCF-7) colorectal carcinoma cell line (HCT-116) and lung adenocarcinoma cell line (A549) in vitro. The fruit and seeds of M. citrifolia were extracted with 95% ethanol and thye filtrate was evaporated by a rotary evaporator. The excess mositure was evaporated and concentrated using vacuum concentrator. The ethanol extract underwent cytotoxicity testing through MTT cell viability assay. The cell viability, assay revealed that all the three human cancer cell lines; lung adenocarcinoma (A549), colorectal carcinoma (HCT-116) and brest adenocarcinoma (MCF-7) exhibited resistance against M. citrifolia fruit ethanol extracts with IC50 values greater than 100 ug/mL. hence, the ethanol extract of M. citrifolia fruits may not serve as anticancer against using MTT assay. Nevertheless, the M. Citrifolia fruits' compounds could be further studied in relation to other phytochemical constituents that could work with chemoprevention. Different cell viability assay could also be utilized in assessing M. citrifolia fruits' anti-proliferative activity and cytotoxicity.