Essential nutrient determination using microwave plasma atomic emission spectroscopy and detection of osysl1 gene among selected CLSU pigmented rices accessions
Date
2019
Authors
Faith C. Manangan
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Abstract
Health is so important to all people, and having a good health is also having a nutritious food in the table. Rice is the most staple food in the Philippines. In this study, all the seed morphology varies according to their variety. Seed morphological characterization of rice was performed specifically the grain length, grain width, grain size and grain shape. The zinc, manganese, copper, iron and cadmium composition of fifteen selected CLSU pigmented rice accession have been analyzed using the Microwave Plasma/Atomic Emission Spectroscopy (MP-AES). gene profiling was performed using the OsYSL1 gene for iron. The nutrient analysis found that Zinc has the range of 13-23mg/kg, for Copper range in 6-13 mg/kg, for Manganese range in 1-13 mg/kg, for Iron range in 52-1071 mg/kg, lastly for the cadmium have equal they are all equal with 0.01 mg/kg which means that there is no cadmium content within the rice accessions. Molecular detection reveals that OsYSL1 gene was present in all selected pigmented rice. However, the functionality of the gene needs a further study.