Mycochemical composition and biological activities of soil borne fungi isolated from onion fiels
Date
2019
Authors
DANNA C. CAMACHO
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Abstract
This study aimed to determine the mychochemical constituents and biological activities of fungi isolated from onion field. Penicillium citrimum, Scopularriopsis brevicaulis and Aspergillus niger.
Mycochemicals such as cardiac glycosides, flavonoids and terpenoids were present in ethanol extracts of S. brevicaulis and P. citrinum. Meanwhile, tannins were present in S. brevicaulis ethanol extract and saponins were detected in A. niger ethanol extract and P. citrimum mycelial spent. Steroids were present in S. brevicaulis mycelial spent. In addition, P. citrimum and A. niger mycelial spent contains cardiac glycosides and A. niger mycelial spent contains caediac glycosides and A. niger mycelial spent also contains flavonoids.
For antibacterial activity as protectant, at 12 hours and 24 hours of incubation, the leastzone of colonization of S. aureus and E. coli were recorded in plates treated with A. niger and S. brevicaulis ethanol extracts (6mm for S. aureus, 7.27 mm for E. coli) and P. citrimum ethanol extract and fungal spent (6.53 mm for S. aureus, 7.57 mm for E. coli) respectively. This suggests antibacterial activity as protectant of P. citrimum, S. brevicaulis and A. niger ethanol extract and spent against S. aureus and E. coli. For the eradicant test, among the six extracts used, S. brevicaulis ethanol extract had the widest zone of inhibition after 12 hours of incubation with 11.20 mm for S. aureus and A. niger spent with 12.82 mm for E. coli, respectively. Furthermore, at 24 hours of incubation a reduction in zone of inhibition was observed in other treatments.
DPPH free radical scabenging assay and total phenolic content revealed low radical scavenging activity and total phenolic content of the fungi isolated from onion field. Among which A. niger registered the highest RSA and total phenolic content of 20.35% and 33.75% respectively.