Cytotoxicity, Antibacterial and Phytochemical analysis of Serpentina (Andrographis paniculata Nees) leaves extract [manuscript]
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Date
2017
Authors
Francesca Jennina V. Jose
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Abstract
Andrographis paniculata Nees is a member of the family Acanthaceae that is commonly used by the local people, for the treatment of common cold, liver diseases, snake bite and some skin infection. In this study, the cytotoxicity, antibacterial activity and phytochemical composition of the leaf extracts of A. paniculata were investigated. The cytotoxicity assessment was determined using brine shrimp assay and lethal concentration (LC50) of the extracts was analyzed using the probit analysis. The antibacterial activity was determined using agar disc diffusion method against Staphylococcus aureus and Escherichia coli. Chemical constituents of the plants were screened using the standard method pf phytochemical analysis. results of our study revealed that cytotoxicity assay of both ethanol and hot water extract of A. paniculata considered as toxic having LC50 of 124.86µg/ml and 116.27µg/ml for both hot water and ethanol extracts respectively. In antibacterial activity, both extracts did not inhibit the growth of both bacterial pathogen used. Results of phytochemical analysis showed that A. paniculate contained saponins, tannins, flavonoids, coumarins found in ethanol extract and tannins, terpenoids, coumarins in hot water extract.