Phytochemical screening and teratogenic effect of Durio zibethinus on Zebrafish (Danio rerio) embryos [manuscript]
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Date
2017
Authors
Janine E. Estanislao
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Abstract
In this study, teratogenic effect of Durio zibethinus plant parts (leaves, stem-bark and fruit rind) were evaluated using zebrafish embryos and phytochemicals screened on the aqueous extract of durian plant parts was done. Higher concentrations such as 2% and 3% in the stem-bark showed coagulation of embryos, as well as in leaves and fruit rind after 24 hours of exposure which is a manifestation of the lethality of the extract. The heartbeat of the embryos in the lower concentrations was significantly lower to the embryos in the control. Phytochemical screening was carried out qualitatively following the protocol described by Sofowora (1993). The study revealed that saponins were found present in the fruit rind and stem bark of the D. zibethinus, while cardiac glycosides were found present in the stem-bark. D. zibethinus stem-bark was the most toxic plant part followed by the leaf then fruit rind. D. zibethinus exhibits teratogenic property and growth retardation was the most notable teratogenic effect of the plant.