Phytochemical screening and determination of the biological activities of Bambusa vulgaris var.striata (Loodd ex Lindel) and Dendrocalamus asper (Schult ex Backer) Shoot extracts [manuscript]
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Date
2017
Authors
Bon Jovi Ramos Baguistan
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Abstract
This study was conducted the presence of phytochemicals such as alkaloids, cardiac glycosides, flavonoids, saponins, steroids, tannins, and terpenoids. Additionally, the antibacterial and anti-oxidant properties of B. vulgaris var. striata and D. asper shoot extractswere also evaluated. The antibacterial property was evaluated using disc diffusion method against two pathogens E. coli and S. aureus. Meanwhile, DPPH radical scavenging assay was used for the determination of the antioxidant activity and the total phenolic content of the shoot extracts. The phytochemical screening of four shoot extracts revealed the presence of cardiac glycosides, flavonoids, terpenoids and saponins. In addition, steroids are also present in ethanol extract of B. vulgaris var. striata and D. asper shoot while tannins were present only in ethanol extract of B. vulgaris var. striata shoots. For the antibacterial activity, the ethanol extract of B. vulgaris and D. asper shoots showed a minimal eradicating activity against E. coli and strong eradicating activity against S. aureus and at 12 and 24 hours of incubation. In addition, ethanol extract of B. vulgaris var. striata showed protectant activity against E. coli and S. aureus at all incubation period. Furthermore, the antioxidant activity of ethanol extract of D. asper showed the highest radical scavenging activity with a mean of 64.80%. Meanwhile, the highest total phenolic content was recorded in B. vulgaris var. striata ethanol extract with 49.90 mg AAE/g sample. The antioxidant and total phenolic content has the potential candidate as a good source of natural antioxidant as they fight against free radicals.