Major practice in microprogation of carnation (Dianthus caryophyllus L.) at Benguet State University [manuscript]
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Date
2017
Authors
Juvy Ann C. Villena
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Abstract
This major practice with emphasis on micropropagation of carnation was conducted from December 22, 2016 to February 17, 2017 at Regional Tissue Culture Laboratory of Benguet State University in Cabanao, La Trinidad, Benguet.
The objectives of the major practice were to know the different aspects of tissue culture and gain knowledge on both experiential and technical areas and to conduct experiments to enable the MP students to : a develop proficiency in inoculation procedures, acquire a more precise ability and technical knowledge in preparing stock solution, gain a hand on experience in mericloning or subcloning of carnation and become familiarize with the ex-vitro acclimatization of plantlets grown from in vitro.
The major practice student experienced laboratory activities such as preparation of stock solution, culture media and explant; inoculation of carnation, African Violet, Chrysanthemum, Orchid pod, Carnation and Chrysanthemum flower bud; and mericloning of carnation and chrysanthemum.
She also experienced working outside the laboratory like collection of explants, transplanting of survived acclimatized plantlets, weeding and sterilizing of the soil medium.