Surveillance and identification of antimicrobial resistant Escheriichia coli and Salmonella in livestock farms, milk and meat in Ilocos Region [manuscript]
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Date
2019
Authors
Christine Hope P. Parcon
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Abstract
The study was conducted to determine the prevalence of antibiotic-resistant Escherichia coli and Salmonella spp. from the livestock and poultry anmals and their products in the Ilocos region. A total of 150 meat samples (pork, beef, and chicken meat), milk and swab samples (environmental, rectal and cloacal) were collected in the wet markets, supermarkets, abattoirs and livestock and poultry farms in seven (7) selected municipalities in Pangasinan namely Alaminos, Mangatarem, Calasiao, San Nicolas, Asingan, Natividad and San Quintin. After bacterial culture, the isolates were subjected to biochemical test for confirmation of Salmonella and Escherichia coli which then underwent antibiotic sensitivity testing in 14 different antimicrobials. Results of bacterial culture and biochemical testing revealed that 53.33% (40/75) of the collected samples were positive to E. coli and 10.67% (8/75) to Salmonella. Antibiotic sensitivity test results showed that E. coli and Salmonella spp. were highly resistant to tetracycline, chloramphenicol, cotrimoxazole, ampicillin, streptomycin and gentamicin. The most frequent resistance pattern found in Ilocos E. coli isolates was AMP-C-COT-K-TET and AMP-TET for Salmonella. From the total number of E. coli and Salmonella sp. isolates, 63.24% and 100% were observed to be multi-drug resistant strains, respectively.