Epiphytic fungi associated in off season tomato [manuscript]
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Date
2018
Authors
Dominic Sanjaya B. Wijayawickrama
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Abstract
Tomato is a widely used crop all over the world but its production is oftentimes confronted by the incidence of diseases caused by plant pathogenic bacteria, viruses and fungi. This study was conducted to monitor the different epiphytic fungi present on the different growth stages of tomato suring its off-season cultivation. Temperature and humidity were also recorded in this study to determine if there is any effect on the growth and population of fungi. The isolated fungi were then classified based on their cultural and morphological characteristics. Identification of species based on their molecular characteristics were carried out by the amplification of DNA ITS region using the PCR with the forward primers ITS1 and reverse ITS 4 and was confirmed by using gel electrophoresis. Basic Local Alignment Search Tool (BLAST) was used for species identification.
There are ten different epiphytic were isolated from the farm. These are Aspergillus falvus, Aspergillus niger, Aspergillus fumigatus, Aspergillus parasiticus, Fusarium solani, Fusarium proliferatum, Penicillum oxalium, Trichoderma atroviride, Acremonium and Rhizopus. Aspergillus flavus and Aspergillus niger amongst all the collected fungi occurred the most.