Functional activities and immunostimulating potential of red seaweeds methanol extraction on nile tilapia against Aeromonas hydrophila / [manuscript]
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Date
2018
Authors
Jhomelyn V. Abelende
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Abstract
Aquaculture practices harbor some bacterial infection, and one of the most common fish pathogens encountered by the fisherfolks is Aeromonas hydrophila. Immunostimulants are currently used as an alternative to the drugs, chemicals and antibiotics to normal fish diseases because in contrast to vaccines,they enhance the innate immune response. The findings of the study revealed that extract of Kappapychus alvarezii contains alkaloids, terpenoids, steroids, flavonoids and quinones whereas, extract of gracilaria spp. contains terpenoids and coumarin, and extract of Galaxaura oblongata contains alkaloids and terpenoid. K. alvarezii, Gracilaria spp., and G. oblongata showed a zone of inhibition of 7.29 mm, 7.17 mm and 7.80 mm respectively. Moreover, K. alvarezii, Gracilaria spp., and G. oblongata had 36.73%, 30.50% and 24.46% radical scavenging activity. The result of the in-vitro assay revealed that it affect the haematological parameters by means of normalizing the RBC, hematocrit and hemoglobin and increasing the production of white blood cell acting as defense mechanism. Furthermore, it reduced the symptoms exhibited by A. hydrophila, lowered the mortality and showed higher percentage of survival excluding for the extract of Gracilaria spp. which had moderate survival and mortality. Generally, the extract of K. alvarezzi, Gracilaria spp, and G. oblongata were efficient to used as immunostimulant for nile tilapia infected with A. hydrophila.