Genotypic variation and estimation of blast resistance genes on backcross inbred lines (US-2*2/Malay 2) using rice blast differential system [manuscript]
No Thumbnail Available
Views
...
Downloads
...
Date
2018
Authors
Karla Trisha F. Alejandro
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Resistance estimate of gene of the BC1F3 mapping population derived from US-2*2/Malay 2 crosses was conducted at PhilRice-CES blast nursery. Two Types of evaluation were performed: leaf infection type evaluation conducted at 35 and 60 days after sowing (DAS) and percent severity evaluation conducted at 35 DAS. For the leaf infection type evaluation, 25% of the mapping population were resistant, 64.53% were susceptible. For percent severity evaluation, 6.98% were resistant, 20.93% were intermediate and 72.09% were susceptible. Comparing the disease reaction of US-2*2/Malay 2 mapping population with the reaction of blast differential varieties, five genes, namely: Pik-m, Pib, Pish, Pish, Pi3, Pil9(t) were estimated to be possibly carried by the population associated to blast resistance. The estimated R genes were validated by inoculating the 15 blast resistant lines with two differential isolates, M101-1-2-9-1 and JMB8401. These two blast isolates have shown differentiating ability against the estimated R genes. Following inoculation with differential isolates, Pik-m, Pib, Pish and Pi3 genes were confirmed to be present in the BC1F3 mapping population of US-2*2/Malay 2.