Evaluation of angiosuppressive activity of Pleurotus florida on developing chick embryo using chorioallantoic membrane assay [manuscript]
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Date
2018
Authors
Rodene C. Zacarias
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Abstract
Angiogenesis is the proliferation of new blood vessels that promote normal embryonic development and numerous pathologies like tumor growth and cancer metastasis. The assays were used for both pro and anti-angiogenic agents. This study evaluated the angio suppressive activity of Pleurotus florida on the developing chick embryo using chorioallantoic membrane (CAM) assay and used destructive sampling into four different treatment concentrations and a control. The result revealed that there is a decrease on the number of blood vessels formed after 24 hours, 48 hours, and 72 hours at a higher treatment concentrations. Statistical analysis showed that the 24 hours and 48 hours of exposure to various concentration have no significant difference. Nevertheless, after 72 hours of exposure, the mean blood vessels formed showed a significant difference among the treatment means between the highest concentration of P. florida (1%) and the control treatment (sterile water). In terms of the percentage angio-suppressive activity of P. florida with the greatest angiogenic effect which was the 1% P. florida treatment concentration possesses 35.63% and 38.65% angio-suppressive rate after 48 hours and 72 hours of exposure respectively. Thus, the study showed that Pleurotus florida lyophilized hot water extract exhibits angio-suppressive activity on the developing chick embryo using chorioallantoic membrane assay and is concentration and time exposure dependent.