zoochemicals and functional activities of sea cucumber (Holothuria leucospilota) in Casiguran, Aurora
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Date
2019
Authors
Job Daniel Noah G. Cruz
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Abstract
The functional activities of Holothuria leucospilota was assessed using different assay. Collection Holothuria Casiguran, Aurora yielded two species. Hothuria leucospilota and Holothuria hilla that belong to Order Aspidochirotida and Family Holothuridae. H. leucospilota is the dominant species and was used in the study and in different functional activities. the body wall of H. leucospilota screened for different chemical components, and among the present compounds are Essential oil, Tritepene, Steroids, Phenols, Coumarines, Anthrones, Tannins and Flavonoids. It was also found out that H. leucospilota possess satisfactory level of antioxidant with 37.50% scavenging effect with phenolic content of 14.00 mg GAE/g of sample.
In addition, embryo toxicity and teratogenecity of H. leucospilota was observed in different concentration in Hot watrer and fresh body wall extract. In the toxicity to the embryo test, result shows that in hot water extract it showed non-toxic while fresh body wall extract in the highest concentration (10,000 ppm), it is toxic. Hot water extract do not have terratogenic effect but a delayed growth was monitored. In fresh body wall extract delayed growth was observed in all treatments and perverted tail in 1000 ppm concentration was seen. Cam irritation Inhibition Assay of H. leucospilota revealed that in 1000 ug/ml shows moderate inhibition. It concluded that H. leucospilota has potential functional activities.