In Vitro and vivo screening of garlic (Allium sativum) leaf extracts against Aeromonas hydrophila on nile tilapia (Oreochromis niloticus L [manuscript]
| dc.contributor.author | Lea Joy D. Darapieza | |
| dc.date.accessioned | 2025-09-10T07:54:26Z | |
| dc.date.available | 2025-09-10T07:54:26Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | The study was done to evaluate the garlic leaf extract as potential alternative antibiotic for Aeromonas hydrophila. The study had two experiments. The first experiment was the in vitro experiment, aimed to determine the susceptibility of A. hydrophila in garlic leaf extract using different extraction solvents. The second experiment was the in vivo experiment, aimed to determine the susceptibility differences in the survival and water quality of Nile tilapia among all treatments. Bacterial plate counting was also done to determine bacterial colonies present in fish. A total 45 Freshwater Aquaculture Center nile tilapia were used in this study. The treatments used for in vitro experiment are antibiotic chloramphenicol (positive control), distilled water (negative control), aqueous extract (T3), ethanol (T4) and methanol (T5). In in vitro experiment, the susceptibility of A. hydrophila in chloramphenicol and garlic leaf extracted in methanol showed no significant difference. However, garlic leaf extracted in ethyl alcohol (T4) and aqueous extract (T3) were comparable to one another but significantly different from chloramphenicol (T1) and garlic leaf extracted in methanol (T5). In in vitro experiment, water quality in all treatments were comparable. In the survival of Nile tilapia, antibiotic chloramphenicol (T1) and aqueous extract (T2) were significant but methanol extract (T3) was not significant as compared with T1 and T2. T1 and T3 were significantly different from T2. Screening of garlic leaves in in vitro and vivo experiment had positive effect on both experiment. This can be used as an alternative antimicrobial rather than antibiotic because it is inexpensive and has less negative impacts to fish and human. It was recommended for future studies to determine the phytochemical constituents present in garlic leaf to know its bioactive compounds, try another species of fish, determine the mode of action of antibiotic in A. hydrophila and use the clinical standards in microbial testing. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/537 | |
| dc.language.iso | en | |
| dc.relation.supervisor | JANET O. SATURNO | |
| dc.title | In Vitro and vivo screening of garlic (Allium sativum) leaf extracts against Aeromonas hydrophila on nile tilapia (Oreochromis niloticus L [manuscript] | |
| dc.type | Thesis |