Evaluation of combined use of Trichoderma and Bacillus as potential biocontrol of Southern Blight on tomato [manuscript]
| dc.contributor.author | Noemi C. Ramilo | |
| dc.date.accessioned | 2026-05-25T06:04:30Z | |
| dc.date.available | 2026-05-25T06:04:30Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | A combination of two biological control agents, Trichoderma sp. and Bacillus subtilis were tested in vitro against Sclerotium rolfsii as potential control for Southern blight in Lycopersicon esculentum Miller. Radical growth of the mycelium of S. rolfsii was inhibited by Trichoderma sp. in dual petri plate assay which may be attributed to fast colonization of the mycelia as well as the production of spores. Microscopic studies showed the hyphae of S. rolfsii surrounded by Trichoderma sp. with subsequent disintegration. Antagonistic activity of Trichoderma sp. was demonstrated by coiling and suppressing the production of sclerotia which limits its activity in vitro. However, the antagonism of B. subtilis against S. rolfsii was not observed on dual petri plate assay. Microscopic studies confirmed lack of evidences as shown in the absence of hyphal interference of B. subtilis against S. rolfsii in vitro. Trichoderma sp. and B. subtilis were subjected to pathogenicity test to determine their antagonistic activity against S. rolfsii on screen house setting. Culture suspension of Trichoderma sp. and B. subtilis were obtained and sprayed on a 150-day old L. esculentum Miller plant. Test plants in negative control exhibited symptoms of Southern blight approximately after 5 days. Reduced symptoms were exhibited on the test plants treated with Trichoderma sp. and B. subtilis. The progression of symptoms slowed down, which needs further testing in screenhouse/field testing. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/1416 | |
| dc.language.iso | en_US | |
| dc.relation.supervisor | ZOSIMO G. BАТТAD II, M. Sc. | |
| dc.title | Evaluation of combined use of Trichoderma and Bacillus as potential biocontrol of Southern Blight on tomato [manuscript] | |
| dc.type | Thesis |