Sequencing and characterization of LAC2 gene of Ganoderma lucidum strain [manuscript]
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Date
2017
Authors
Darlina P. De Vera
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Abstract
The partial sequence of GL_LAC2 from available mycelia of G. lucidum was isolated in this study. The amplified product have 1.5 kb region with 559 bp using primer LacF1 and LacR1. The resulting sequence was extracted using Geneious v.9.05 ad De Novo assemble the consensus sequence of the partial amplified LAC2 gene. Meanwhile, the GL_LAC2 have shown the closet resemblance with Ganoderma weberianum with at 67% confidence level, followed by Ganoderma lucidum and Ganoderma tsugae based on Phylogeny analysis which suggest that multiple genes evolutionarily derived from different ancestral laccase genes and phylogenetic structure. The GL_LAC had 84.18 % similarity with LAC2 gene of G. weberianum (KF384100.1), particularly exhibiting 115 number of polymorphism sites, indicating the uniqueness and diveristy of this gene among the said strain. On the other hand, the GL_LAC2 of G. lucidum had 79.56% similarity with G. lucidum (DQ914869.1), having 246 polymorphism indicating distant relativity or few conservation among the target region. This study compared the laccase gene of G. lucidum to other Ganoderma sp. found in NCBI to determine the diversity and availability of sequences.