Isolation and molecular identification of microalgae collected in Minalungao National Park, Nueva Ecija.
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Date
2020
Authors
Marisol M. Baltazar
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Abstract
Microalgae are diverse group of prokaryotic and eukaryotic photosynthetic microorganisms that grow rapidly in a simple and efficient way. This study was conducted to collect, siolate and molecularly identify the microalgae in Minalungao National Park, Nueva Ecija. Microalgae cultures were incubated with temperature of 27°C-30°C with an aerator and urea to promote the growth of microalgae. isolation of microalgae was identified using morphological and molecular approach by PCR using rbcl.primer.
Two species of microalgae were collected and siolated from Minalungao National Park. They were identified as Deserella yichangensis (94.46% identity) and Chlorophyta sp. (99.18% identify) based on the BLAST analysis. The result of phytochemical screening showed the presence of active components such as essential oils, triterpenes, phenols, fatty acids, anthraquinones, tannins, flavonoids, alkaloids, steroids and coumarins in D. yichangensis and Chlorophyta sp. On the other hand, anthrones is absent in D. yichangensis and sugars in Chlorophyta sp.