Effect of ethanol extract of wild fruiting body of Trametes suaveolens on the food intake and locomotion of N2 wild strain Caenorhabditis elegans
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Date
2019
Authors
Leomark N. Parocha
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Abstract
Trametes suaveolens is one of the macrofungi that grwos on rotting log. This study evaluated the effects of fruiting body extract of T. suaveolens on the food intake and locomotion of Caenorhabditis elegans. The active components of mushroom were obtained using ethanol as solvent. The nematode lethality assay, pharyngeal behavior assay and locomotion assay were determined after exposure to different concentrations of the extract. A 1000 ug/mLextract was found to be moderately roxic at 120 hours post treatment exposure (hpte). At the same concentration, pumping rate was reduced having 59 pumps per minute at 48 hours post-treatment and 15.17 pumps/minute at 120 hpteand decreased to 2.5 reversal/minute at 120 hpte. On the other hand, in roaming locomotion, 1000 ug/mL exposed nematodes recorded 22.523 body bends/minute at 48 hour and increased to 24.33 body bends/minute at 120 hpte.
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Research Subject Categories::NATURAL SCIENCES::Biology , Research Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Microbiology