Phytochemical screening, Embryo-Toxic and Teratogenic effect of Persea americana Extract on Zebrafish (Danio rerio) embryos [manuscript]
| dc.contributor.author | Klea NiƱa P. Baluyot | |
| dc.date.accessioned | 2026-05-21T01:48:40Z | |
| dc.date.available | 2026-05-21T01:48:40Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | The evaluation of embryo-toxicity and teratogenicity activities of P. americana leaves, fruit rind and stem bark HWEs using zebrafish embryos as animal experimental model was conducted in this study. Mortality and hatchability of embryos as animal exposed to different concentrations. Meanwhile, the heartbeat was only examined at pharngula stage. Furthermore, the morphological abnormalities of zebrafish embryos were recorded at 48-72 hpta. Test tube methods following the protocol of Sofowara (1993), Trease and Evans (1989) and Harborne (1973) were used for the phytochemical analyses. Various phytochemical, including tannins, saponins, flavonoids and terpenoids, are present in the leaf, stem-bark and fruit rind of P. americana. A 100% mortality was observed at 1% or higher concentration of leaf and stem-bark and at 0.5% of fruit rind at 48 hpta. LC50 values are estimated using a probit regression analysis. P. americana leaves, stem-bark and fruit rind extracts showed LC50 values of 562,774 and 348 mg/ml respectively. No hatchability was observed in 0.5% and higher concentrations of P. americana leaf, stem-bark and fruit rind HWEs. Only 25% of hatchability was noted in leaf extract at 0.1% concentration whereas a 75% hatchability was recorded in 0.1% fruit rind HWE and 83.33% hatchability was observed in leaf HWE in the same concentration. In terms of heartbeat, 1% and higher concentrations of P. americana HWEs had no heartbeat since 100% mortality was observed after 36 hours of exposure. Generally, coagulation and absence of heartbeat were the observed toxic effects. Growth retardation, limited movement, malformation of head and tail were the most observed teratogenic effects of P. americana leaves, stem-bark and fruit rind HWEs to the developing embryos of zebrafish. These significant effects of the plant HWEs strongly indicate their promising potential as source of anticancer agents. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/1355 | |
| dc.language.iso | en_US | |
| dc.relation.supervisor | EDEN S. DAVID Ph.D. | |
| dc.title | Phytochemical screening, Embryo-Toxic and Teratogenic effect of Persea americana Extract on Zebrafish (Danio rerio) embryos [manuscript] | |
| dc.type | Thesis |