Assessment of sperm Kinematics of sexed cattle (Bos taurus) semen sorted using commercial sexing kit
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Date
2019
Authors
Carl Isiah S. Bernardo
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Abstract
Semen sexing is one of the method utilized nowadays in order to increase production of productive animals of the desired sex to combat problems in milk and meat scarcity due to increasing population. Producers claimed that commercial semen sexing agent hyper stimulate the desired sperm to fertilize the egg. Hence, this study aimed to determine the motility and kinematic parameters of sexed cattle semen sorting using Bull focus (BF) and Heifer Focus (HF) incubated at 5°C and 37°C in varying incubation time in CASA. Mixed Model ANNOVA was applied to determine interaction between incubation time and treatment.
Interaction betweeen incubation time and treatment (semen treated with BF and control) was significant (p<0.05) in most kinematic parameters except STR, LIn and wobble WOB at 37°C and TM, VAP, VCL and BCF at 5°C. Furthermore, interaction effect was not observed in HF and control at 37°C and there is significant interaction effect (p<0.05) between incubation and treatment (HF and control) in TM, PM and all sperm kinematic parameters. the study showed highest total motility (TM), progressive motility (PM) and sperm kinematic parameters at 15 mins of incubation for 37°C and 2 hrs for 5°C for both semen treated with Bull Focus and Heifer Focus, however, significant differences was not present (p>0.05). Moreover, there is a significant decrease in TM, PM and sperm kinematic parameters of semen treated with Bull Focus and Heifer Focus incubated at 5°C starting at the 12hrs to 36hours of incubation.