Detection of Abortifacient bacterial agents of water buffaloes (Bubalus bubalis) in selected cities and municipalities of Nueva Ecija [manuscript]
| dc.contributor.author | Noel M. Salvador | |
| dc.date.accessioned | 2025-06-13T00:17:12Z | |
| dc.date.available | 2025-06-13T00:17:12Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | The detection of presence of abortifacient bacterial agents in Nueva Ecija was the focus of this study. Specifically, it detected the presence of abortifacient bacterial agents’ Leptospira sp. And Brucella sp. in buffalo within selected cities and municipalities of Nueva Ecija with history and/or current abortion cases using nested Polymerase Chain Reaction (nPCR) and Rose Bengal Plate Test (RBPT), and determined the positive rate of Leptospirosis and Brucellosis in Nueva Ecija. A total of one hundred (100) serum samples and one hundred (100) urine samples from randomly selected buffaloes were collected for this study. Urine samples for Leptospira sp. were subjected to DNA extraction protocol and amplification using nPCR assay. The nPCR product were subjected to gel electrophoresis and viewed under UV transilluminator to confirm the presence of DNA bands lined at approximately 730 base pair. Serum samples for Brucella sp. were subjected RBPT, and was observed for the presence of antibody through agglutination reaction. Results revealed that the positive rate of Leptospirosis in Nueva Ecija was 7%. The positive animals identified were from the municipality of Carranglan (3/15; 20%), Science City of Munoz (2/14; 14.28%) and San Jose City (2/15; 13.33%). The positive rate of Nueva Ecija for Brucellosis was 15.39% (2/13) in which, only General Mamerto Natividad has shown a positive result. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/259 | |
| dc.language.iso | en_US | |
| dc.subject | Research Subject Categories::VETERINARY MEDICINE | |
| dc.title | Detection of Abortifacient bacterial agents of water buffaloes (Bubalus bubalis) in selected cities and municipalities of Nueva Ecija [manuscript] | |
| dc.type | Thesis |