Molecular identification and phytochemical profiling of microalgae using thin layer chromatography [manuscript]
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Date
2019
Authors
Kassandra Jlin B. Mariano
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Abstract
The study was focused on the molecular identification of the microalgae and evaluation of the phytochemicals it contain through thin layer chromatography.
The genomic DNA was extracted and was subjected to PCR amplification using nrDNA ITS and rbcL gene markers. The sample was amplified by using ITS gene marker. After the sequencing procedure, it was revealed in the BLAST analysis the sample has 95% similarity to Dictyosphaerium sp. and was found out that the morphological characters of the two conform. The similarity of the morphological characters of the two supports the successful molecular identification of the microalgae with the use of ITS gene marker.
On the other hand, the results of the phytochemical screening revealed that phenols, steroids, triterpenes, essential oils, anthraquinones, coumarins, anthrones, phenolics compounds, tannins, alkaloids and flavonoids were present in one sample and the other was also positive for all the constituents tested except for tannins. the phytochemical screening showed that the microalgae sample contain phytochemicals that have great importance in the medicinal field which can be used for future studies.