Isolation and characterization of mungbean rhizobia grown from slected soil in Eastern Pangasinan [manuscript]
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Date
2017
Authors
Bethel Charis C. Elevazo
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Abstract
This study was conducted using 30 soil samples collected from 10 municipalities in Eastern Pangasinan. An experiment was conducted to evaluate the relatedness of nodule population with particle size distribution and organic matter. Also to identify probable rhizobium species using DNA analyses.
Correlation results showed that nodule weight and plant weight; number of nodule and soil organic matter, % Clay and weight of nodule; % silt and number of nodule; % sand and number of nodule has a significant effect.
Morphological characteristics of rhizobia has a circular shape, faintly pink to white in color, 0.5 mm to 3 mm in size and has a translucent opacity. There are 15 acid producing rhizobia, 9 alkaline producing rhizobia and 6 contaminated isolates out of 30 isolates. The acid producing bacteriaare the fast growing rhizobia called Rhizobium which was observed 2-3 days after inoculation while the alkaline producing bacteria are the slow growing rhizobia called Bradyrhizobium which was observed 6-8 days after inoculation.
there were 24 out of 30 isolates that were subjected to Molecular DNA analyses. Out of 24 samples, there were only 3 amplicons, MAN2 (A), MANG2 (5C) and SIS2 (17C).
Based on the findings of the study, it can be concluded that mungbean plants can be infected both by Bradyrhizobium and Rhizobium. Rhizobium and Bracdyrhizobium bacteria re present in any textural class. Moreover, acid producing Rhizobium is more dominant that Bradyrhizobium in acid soils.