In vitro antimicrobial activity of crude extract of fecal bacteria from Philippine native Pig (Sus scrofa) Against bacteria isolated from diarrheic piglet [manuscript]
| dc.contributor.author | Patricia E. Morillo | |
| dc.date.accessioned | 2026-04-28T00:21:31Z | |
| dc.date.available | 2026-04-28T00:21:31Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Crude extracts of fecal bacteria from Philippine native Pigs were evaluated for in vitro antimicrobial activity against bacteria from diarrheic piglets. This study measured and compared zones of inhibition from crude extracts produced at different incubation periods (6, 12, 18 and 24h) and from commercially produced antimicrobials. A 100µl feed solution were placed on nutrient agar plate then incubated for 24 hours at 37°C. 12 isolates were produced and characterized morphologically based on colony morphology and Grain stain. Two distinct colony morphologies were formed, raised and wrinkle-patterned surface and flat and mucoid surface. Gram staining showed all isolates are positive and rod-shaped. Two isolates (S1-1 and S2-3) with different colony morphology were randomly selected as test bacteria A and test bacteria B for antimicrobial activity. Filter paper discs, impregnated with previously prepared 30µl of crude extracts, and antimicrobial discs were evenlly placed on Mueller-Hilton agar plate inoculated with the test bacteria, then incubated for 18h at 37°C. Zone of inhibition were measured. Three zones of inhibition were produced against test bacteria A, and six against test bacteria B. Both test bacteria are resistant to amipicillin and TMPS, but susceptible to chloramphenicol, ciprofloxacin, clindamycin, enrofloxacin, erythromycin, and gentamicin. Zones of inhibition produced by the crude extracts are statistically insignificant with one another, but significantly higher than ampicillin and TMPS. | |
| dc.identifier.uri | http://granarium.clsu.edu.ph/handle/123456789/1189 | |
| dc.language.iso | en_US | |
| dc.relation.supervisor | FROILAN BERNARD R. MATIAS, DVM, MSc | |
| dc.title | In vitro antimicrobial activity of crude extract of fecal bacteria from Philippine native Pig (Sus scrofa) Against bacteria isolated from diarrheic piglet [manuscript] | |
| dc.type | Thesis |