Extraction of female crude sex pheromone of onion armyworm Spodoptera exigua Hubner (Lepidoptera : Noctuidae) and its attractancy effect [manuscript]
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Date
2019
Authors
Ryan Alexis P. Bautista
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Abstract
The study was conducted to evaluate different solvents in extracting crude sex pheromones in female onion armyworm to determine the attractancy effect of the crude sex pheromone to male armyworm moths under laboratory condition and to test the efficacy of the extracted crude pheromones hormones under field condition. the protocol for extraction was based on Cayabyab (1997). The eight and ninth segments of the abdomen of the female armyworm consisting the pheromone gland were exised, then soaked in solvent (Acetone, Hexane and Ethanol), then placed with rubber septum and crude pheromone was extracted. The attractancy test was done using no choice method and 20 active male moths (one to two day old) were used per replication. The percent attracted male moth was recorded at 5 min, 10 min, 15 min, 20 min, 25 min and 30 mins after releases. Treatment 3 (Hexane 1:1) was found significantly attractive on the male moth under laboratory condition after 30 min of observation time with 30% attractancy and Treatment 6 (Ethanol 1:2) was the least with 11.67% attractancy. Number of male moth caught per trap was monitored daily for one week. Also, number of armyworm larval and damage rating were recorded from 30 sample onion plants.