Allelopathic tannins from guava(Psidium guajava L.) leaf extracts against the rice weed, Echinochloa crus-galli [manuscript]
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Date
2017
Authors
JASMIN SALVADOR PAGADUAN
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Abstract
Background: The application of plant allelopathy has been suggested for weed management to minimize the use of synthetic herbicides. This study aimed to extract tannins from Psidium guajava L. and determine its allelopathic effect on the seed germination and seedling growth of Echinochloa crus-galli rice weed. Methods: The dried powdered leaves of Psidium guajava L. were extracted using 80% ethanol. The crude extract was further extracted done on the leaf extract and fractions of Psidium guajava L. and the total tannin content was also obtained. The allelopathic effect of the tannin from Psidium guajava L. on the seed germination and seedling growth of Echinochloa crus-galli was evaluated. The tannin was characterized using UV-Vis and IR spectrophotometry. Results: The total tannin content of the leaf extract was found to be 50.9487±0.00 mg GAE/g dry weight. The percent germination of Echinochloa crus-galli was recorded to be 76.67±4.71% in the control and 0 in all treatments. The tannin from Psidium guajava L. significantly affected the seedling growth of Echinochloa crus-galli as indicated by a reduction in the elongation of the seedlings. However, the inhibitory effects of the tannin concentrations were not significantly different from each other but significantly different from the control. The probable tannin present in the leaf extract could be a gallotannin based from the UV-Vis and IR spectra. Conclusion: The tannins from Psidium guajava L. leaf extract has allelopathic effect against Echinochloa crus-galli by inhibiting the seed germination and seedling growth. It has the potential as a weedicide.