Cytological properties of nano Allicin
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Date
2019
Authors
Kelvin Louie D. Ancheta
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Abstract
This study was conducted to assess the cytological effect. Allicin and Nano Allicin using three different bioassays (teratogenicity, cytotoxicity, and genotoxicity). The Nano allicin were synthesized by performing biological synthesis method, which used silver nitrate as a cross linking agent. The successful synthesized of nano allicin was been validate by the FTIR (Fourier Transform Infrared) analysis.
Based on this study, three different assays were used to examine the possible cytological effects of allicin and nano allicin. Teratogenic effect of allicin and nano allicin were positive in some toxological parameters in lethal effect such as coagulation and no heartbeat also in scoliosis. In addition, Allicin and nano allicin showed negative results in all of toxicological parameters such as tail and head malformation , growth retardation, etc. Allicin and Nano allicin with 10000 ppm concentration (highest concentration evaluated) showed the highest percent mortality. Hearbeat rare of allicin and nano allicin ranges from 162-1800 beats per minute. Hatchability of 100 percent was displayed an allicin concentration of 10 ppm, 1 ppm and the control while nano allicin displayed100 percent of hatchabilityy at concentration of 100 ppm, 1 ppm and the control. Meanwhile, cytotoxicity testing of allicin and nano allicin gives off LC50 of 33.107 ppm and 38.010 ppm, respectively, indicating that both allicin and Nano allicin were toxic substance. And lastly, the genetoxicity assay was not determined using Allium cepa assay.