Isolation and molecular identification of bacteria from partially composted rice straw with cellulose degradation potential [manuscript]
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Date
2017
Authors
Jenilyn C. Gajes
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Abstract
The purpose of this study was to identify the pre-isolated bacterial species potential for cellulose degradation present in partially composted rice straw samples using molecular techniques. Also, to evaluate and confirmed the cellulose degrading ability of the pre-isolated bacterial species. Bacterial species present in partially composted rice straw was isolated using CMC assay. The bacterial species with cellulose degradation potential was identified using molecular assay approach. During the preliminary cellulose assay screening , there are three (3) isolates that showed clear zone around it during the confirmation. Colony PCR using 16S universal primer followed by sequencing and BLAST analysis identified the isolate as Pseudomonas sp. (KF542910.1). Literature suggest that this species found to have a potential for degrading cellulose and has many used in biotechnological processes. Results indicated that the bacterial species identified as pseudomonas sp. was confirmed to degrade cellulose after C<C assay. The importance and usefulness of these bacteria in the remediation of environment needs to be validated.