Extraction of sugar from corn (Zea mays) stalk using carabao rumen fluid hydrolysis [manuscript]
Loading...
Views
1
Downloads
4
Date
2019
Authors
Maria Simonne T. Oliveria
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
This study aimed to extract sugar from corn stalk using 1%, 5%, and 10% carabao rumen fluid in the hydrolysis process for 3 days. Specifically this study aimed to extract fermentable sugar from corn stalk using enzymatic hydrolysis; determine the effect of different concentrations of carabao rumen fluid in the sugar concentration of the hydrolisate after the process of hydrolysis; and determine the amount of fermented sugar after the fermentation process. A 6 liter capacity plastic container was loaded with 300g of washed, sun dried, and shredded corn stalk, together with this is 6 g of urea, 3 L of water and the corresponding concentration of rumen fluid for each treatment, the hydrolysis process was done for 3 days. Yeast fermentation was conducted for 14 days at a room temperature. Evaluation for the sugar concentration of the hydrolysate of corn stalk using carabao rumen fluid shows that using 1%, 5%, and 10% CRF had significant effect on each other. Mean comparison shows treatment with 1% CRF had the lowest amount of sugar concentration or hydrolyzed sugar being 3.93°Bx and significantly different compared to 5% and 10% with corresponding sugar concentration of 4.63°Bx and 5.93°Bx, respectively. For the sugar concentration of the hydrolysate after fermentation, the treatment with 10% CRF has the highest amount of sugar converted to ethanol. 1% CRF has the lowest amount of remaining sugar after fermentation with a value of 1.43°Bx, followed by 5% CRF with 1.73°Bx, and 2.93°Bx for 10% CRF. In conclusion, carabao rumen fluid was a potential source of enzymes that may be used in extracting fermentable sugar from corn stalk that may be used in producing bioethanol, the 10% concentration of carabao rumen fluid was a potential concentration level to produce 5.93°Bx of sugar concentration from hydrolyzed corn stalk, and at 10% CRF the highest amount of fermented sugar reduction was obtained using 10g per liter of yeast.