Inhibitory effect of powdered avocado leaves and crude extract on the germination and growth of Echinochloa crus-galli
Loading...
Date
2019
Authors
Loidel E. Gagelonia
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The principles of allelopathy allow the synthesis of herbicides from the plant secondary metabolites, which is beneficial for sustainable weed management. This study evaluated the inhibitory effect of the powdered sample and crude extract from avocado (Persea americana)leaves on the seed germination and seedling growth of barnyard grass (Echinochloa crus-galli)in vitro and in vivo. The crude extract from avocado leaves was prepared using 85% ethanol. The determination of phenolic compounds was done using Ferric Chloride test for tannins, Dragendorff test for alkaloids, and Shinola test for flavonoids. Total phenolic content was determine using Folin-Ciocalteu assay. Weedicide bioassay was done to determine the inhibitory activity of powdered sample and crude extraction the seed germination and seedling growth of E.crus-galli. Phytochemical tests for phenolic compounds determined the presence of tannins and flavonoids on the crude extract from avocado leaves. The calculated phenolic content from the Folin-Ciocalteu assay was 356.43≠27.87 ug GAE per mg of dry extract. The powdered sample and crude extract from avocado leaves inhibited the seed germination and seedling growth of E.crusgalli in all the concentrations used (1,3,6, and 10ppm).The powdered sample and crude extract from avocado leaves had inhibitory effect on the seed germination and seedling growth of Echinochloa crus-galli, therefore avocado leaves are a potential weedicide.