Morphological and molecular identification of bracket macro fungi Brgy. Pugaro, Balungao, Pangasinan [manuscript]
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Date
2019
Authors
Camille Joy M. Sanchez
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Abstract
Bracket macrofungi are non-timber forest inhabitants that play crucial ecological roles and perform enormous economic advantages for mankind. Hence, the study characterized the bracket macrofungi by both morphological and molecular approaches using both internal transcribed spacer (ITS) sequences and large subunit LSU rDNAs primers. The genomic DNA was extracted using cethyl-trimethyl ammonium bromide (CTAB) method and diluted 1:100 by means of sterilized distilled water and quantified using Multiskan FC Microplate Photometer.
BLAST analysis of nucleotide sequences of the six bracket macrofungi revealed that Gyrodontium sacchari with 99%, Xylaris papulis with 100%, Stereopsis radicans with 91%, Polyporus sp. With 99%, Phlebia sp. With 93% and Ganoderma lucidum with 100% pair wise identity with samples deposited in Genbank (NCBI). Six the collected bracket macrofungi were identified up to species level.