Acetogenic and acid utilizing bacterial content analysis of forestomach fluid of water buffalo (Bubalus bubalis) calves [manuscript]

dc.contributor.authorRoche Zhes G. Robeniol
dc.date.accessioned2026-05-25T06:06:04Z
dc.date.available2026-05-25T06:06:04Z
dc.date.issued2017
dc.description.abstractMicrobial digesta of water buffaloes was investigated to isolate, characterize and differentiate the morphological and cultural analysis of acetogenic and acid utilizing bacteria present from the digestive tract of calves. This is done through feeding with associated different diets upon the calves. The alteration of the feeds diet (raw milk and supplemented feeds diet) from the birth of the calves until the 30th was been observed in the interval of four samplings (day1, 6th, 16th, 16th and 30thy). Samples was isolated anaerobically through anaerobic chamber in avoidance with exposure to environment then incubated at 37degrees Celcius for 24-48 hours for visible growth of microorganisms. The identified genera of bacteria are Oscillospira sp, Ruminococcus sp. and Acetobacterium sp. as the acetogens and acid utilizers are Sarcina sp., Salmonella sp., Streptococcus sp., Desufovibrio sp., Micrococcus sp., and Strepto bacillus sp. The bacterial population is Consistently increasing as the calves grow older. There is no significant difference between the two diets fed for the weaning calf which concludes that replacement of diet is not affected in the weaning of calves. In proximate analysis of feeds, it has total fiber of 8.50%, total protein of 22.53%, total fat of 7.09% and ash with 6.41% while the component of Pakchong variety of grass fed to the weaning calves has an appropriate percentage of 58%-61% of hemicelluloses (NDF), has 7%-11% crude protein and 8%-12% ash. Calves ability to digest feeds began as their rumen is modified throughout their weaning which leads to the development of their monogastric stomach into a modified rumen. However, starter feeds and grass contributes to the quality of the rumen that stimulates the environment and invigorates the ability of the rumen to conforms growth and digest more.
dc.identifier.urihttp://granarium.clsu.edu.ph/handle/123456789/1417
dc.language.isoen_US
dc.relation.supervisorCYNTHIA C. DIVINA, Ph. D.
dc.titleAcetogenic and acid utilizing bacterial content analysis of forestomach fluid of water buffalo (Bubalus bubalis) calves [manuscript]
dc.typeThesis
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