Identification and characterization of fungi present in the digestive tract fluid of water buffalo calves (Bubalus bubalis) fed with two diets [manuscript]
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Date
2017
Authors
Angelli M. Lloranda
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Abstract
The study was conducted to identify and characterize the fungi present in the digestive tract fluid of water buffalo calves from birth to 30 days to weaning fed with two diets through morphological and cultural analysis. As a result, a total of four (4) anaerobic fungi namely: Piromyces sp., Neocallimastrix sp., Caecomyces sp. and Orpinomyces sp. were identified by using modified orpin's media and the Hungate roll tube method which involved a series of sub-culturing of fungal sections on solid media and subsequent culturing in broth and a thorough observation on the morphological and cultural characteristics of the obtained isolates. Consequently, the presence of fungal colonies, gradually increased and was directly proportional to the calf's age in days. This increasing trend was observed regardless of the given feeding treatment. Comparison between the two diets given revealed that the fungal populations did not have any significant difference with respect to the given diets. In conclusion, the population of anaerobic fungi was not affected by the feeding treatments whether milk, feeds and grass or milk and grass only. The survival of the anaerobic fungi is dependent on the fiber content of the diets given which was analysed through proximate analysis.