HSP70 DNA Sequence variation in genetically enhanced tilapia strains of farmed tilapia (Oreochromis niloticus)
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Date
2019
Authors
Lauren Christian E. Ger
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Abstract
The HSP70 is considred as most conserved member of Heat Shock Proteins that is regfulated during thermal stress. It is an ATP-dependent molecular chaperone that participates in wide range of cellular processes especially in protein folding and remodelling. HSP70 has been verified to govern in fish during stress and in the occurrence of damaged protein. In this study six genetically enhanced tilapia strians (GIFT, GET, EXCEL, iBEST, Red tilapia-BFAR, Red tilapia-FAC CLSU and FaST) have been used. Using maximum parsimony, maximum likelihood and neighbor-joining showed the hyperdiversity of FaST strain on other tilapia strains that may assume that it will perform differently from other strains. The other five strains (GIFT, iBEST, Red tilapia-BFAR, Red tilapia-FAC CLSU and GET EXCEL) showed no variation from each other. Also the separation of clades between genetically enhanced tilapia strains and their founder stocks. The branch length of genetically enhanced strains was always extended. This also revealed that there is no significant difference in terms ofof HSP70 DNA sequences from each other and indicates that they have an equal potential to completely adapt to climate change especially in intense heat.