Field practice report on the morphometry and parasitological profiling of different shellfishes and molecular techniques done in fisheries biotechnology center, Science City of Muñoz, Nueva Ecija Philippines
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Date
2017
Authors
Aries Paul D. Padron.
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Abstract
The field practice was conducted at the Fisheries Biotechnology Center (FBC) located in Bureau of Fisheries and Aquatic Resources-National Freshwater Fisheries Technology Center (BFAR-NFFTC) at CLSU compound in Science City of Muñoz, Nueva Ecija from June 13, 2016 to July 16, 2016. The field practice constitutes two main topics; the morphometry and parasitological profiling of different shellfishes sourced from different areas in Luzon and emphasis on the molecular techniques done in FBC. The first topic was part of the research project conducted by FBC entitled "Identification and diagnosis of parasites in high valued shellfishes in the Philippines as a biological reference for their management and control" while the latter was a basic techniques done in FBC for any related molecular activities. For the morphometry and parasitology of shellfish, samples were sourced from different coastal towns in Luzon and were brought to the FBC laboratory for examination. Initially, morphometric parameters of the samples were recorded, and were followed by the dissection of the internal organ for parasitological examination using Olympus stereomicroscope. Normally, the shell surface was also subjected to such examination. Parasites found in samples were preserved in vials containing 96% ethanol. Moreover, molecular activities done in FBC constitute the following: DNA extraction, polymerase chain reaction (PCR) and gel electrophoresis which were discussed in details at the latter part of this report.